SV40-TRANSFORMED HAMSTER CELLS RESISTANT TO 100-250/tg/ml OF ETHIDIUM BROMIDE
نویسندگان
چکیده
SV4O-transformed hamster cells were selected for resistance to ethidium bromide (EB). Several cell lines were established, which grew in the presence of up to 250 /tg/ml EB. The EB resistance is genetically stable. The cloned resistant cells show no difference in morphology, with the exception of the mitochondrial ultrastructure, which exhibits condensed cristae formation. The tumorigenicity of these cells in Syrian gold hamsters is considerably reduced. Incorporation of radioactive labelled thymidine into mitochondrial DNA is not influenced by the presence of the drug. Gel electrophoresis with mitochondrial proteins from, wild-type and resistant cells reveals significantly different patterns. The mechanism of EB resistance is discussed.
منابع مشابه
Establishment and Characterization of Ethidium Bromide Resistance in Simian Virus 40-transformed Hamster Cells
This study describes the isolation and subsequent characterization of four mammalian cell lines resistant to ethidium bromide (EB). Treatment of the simian virus 40- (SV40) transformed hamster cell line F5-1 first led to the establishment of the F2 cell line, which is resistant to 2 microg EB/ml. At this concentration cytochromes c and b are present in almost normal or only slightly diminished ...
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